Journal
FEBS LETTERS
Volume 589, Issue 5, Pages 639-644Publisher
WILEY
DOI: 10.1016/j.febslet.2015.01.029
Keywords
Promiscuous peptide; Antimicrobial; NMR structure; SDS micelles; Green coconut water; Cn-AMP1
Funding
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Fundacao de Amparo a Pesquisa do Estado de Goias (FAPEG)
- Fundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia (FUNDECT)
- Financiadora de Estudos e Projetos (FINEP)
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Multifunctional and promiscuous antimicrobial peptides (AMPs) can be used as an efficient strategy to control pathogens. However, little is known about the structural properties of plant promiscuous AMPs without disulfide bonds. CD and NMR were used to elucidate the structure of the promiscuous peptide Cn-AMP1, a disulfide-free peptide isolated from green coconut water. Data here reported shows that peptide structure is transitory and could be different according to the micro-environment. In this regard, Cn-AMP1 showed a random coil in a water environment and an alpha-helical structure in the presence of SDS-d(25) micelles. Moreover, deuterium exchange experiments showed that Gly4, Arg5 and Met9 residues are less accessible to solvent, suggesting that flexibility and cationic charges seem to be essential for Cn-AMP1 multiple activities. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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