Journal
FASEB JOURNAL
Volume 29, Issue 12, Pages 4815-4828Publisher
WILEY
DOI: 10.1096/fj.15-272963
Keywords
GFAP; Muller glia; electroretinogram; Kir channels; retinal ischemia
Categories
Funding
- VELUX Stiftung
- Crown Princess Margareta's Committee for the Blind
- Stiftelse for Synskadade i fd Malmohus Lan
- Thorsten och Elsa Segerfalks Stiftelse
- European Union [020235-NEUROTRAIN, 512036-GENORET, 237956-EduGlia, 279017-TargetBraIn]
- Deutsche Forschungsgemeinschaft [RE 849/16-1, SPP 1757, KFO134-Se837/5-2, Se837/6-2]
- PRO RETINA-Stiftung
- Center of Integrative Neuroscience [EXC 307]
- Center of Excellence of the German Research Council
- Bernstein Center for Computational Neuroscience, Tubingen
- Swedish Medical Research Council [11548]
- Avtal om Lakarutbildning och Forskning (ALF) Gothenburg [11392]
- Arbetsmarknadens Forsakringsaktiebolag (AFA) Research Foundation
- Soderbergs Foundations
- Sten A. Olsson Foundation for Research and Culture
- Hjarnfonden
- Hagstromer's Foundation Millennium
- NanoNet COST Action Grant [BM1002]
Ask authors/readers for more resources
Vimentin (Vim) and glial fibrillary acidic protein (GFAP) are important components of the intermediate filament (IF) (or nanofilament) system of astroglial cells. We conducted full-field electroretinogram (ERG) recordings and found that whereas photoreceptor responses (a-wave) were normal in uninjured GFAP(-/-)Vim(-/-) mice, b-wave amplitudes were increased. Moreover, we found that Kir (inward rectifier K+) channel protein expression was reduced in the retinas of GFAP(-/-)Vim(-/-) mice and that Kir-mediated current amplitudes were lower in Muller glial cells isolated from these mice. Studies have shown that the IF system, in addition, is involved in the retinal response to injury and that attenuated Muller cell reactivity and reduced photoreceptor cell loss are observed in IF-deficient mice after experimental retinal detachment. We investigated whether the lack of IF proteins would affect cell survival in a retinal ischemia-reperfusion model. We found that although cell loss was induced in both genotypes, the number of surviving cells in the inner retina was lower in IF-deficient mice. Our findings thus show that the inability to produce GFAP and Vim affects normal retinal physiology and that the effect of IF deficiency on retinal cell survival differs, depending on the underlying pathologic condition.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available