Journal
ARCHIVES OF VIROLOGY
Volume 163, Issue 4, Pages 1057-1061Publisher
SPRINGER WIEN
DOI: 10.1007/s00705-018-3706-0
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Funding
- Bill & Melinda Gates Foundation grant titled Yam Improvement for Income and Food Security in West Africa
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A closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for the detection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.). The assay uses a set of six oligonucleotide primers targeting the YMV coat protein region, and the amplification products in YMV-positive samples are visualized by chromogenic detection with SYBR Green I dye. The CT-RT-LAMP assay detected YMV in leaf and tuber tissues of infected plants. The assay is 100 times more sensitive in detecting YMV than standard RT-PCR, while maintaining the same specificity.
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