4.2 Article

Phase modulation nanoscopy: a simple approach to enhanced optical resolution

Journal

FARADAY DISCUSSIONS
Volume 177, Issue -, Pages 507-515

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c4fd00158c

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Funding

  1. EPSRC
  2. ERC
  3. Royal Society URF

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A new modular super-resolution technique called Phase Modulation Nanoscopy (PhMoNa) has been developed in order to break the optical diffraction barrier in Confocal Laser Scanning Microscopy (LSCM). This technique is based on using spatially modulated illumination intensity, whilst harnessing the fluorophore's non-linear emission response. It allows experimental resolution in both lateral and axial domains to be improved by at least a factor of 2. The work is in its initial phase, but by using a custom built Electro Optical Modulator (EOM) in conjunction with functionalised Ln(III) complexes as probes, a sub-diffraction resolution of similar to 60 nm was achieved of selected cellular organelles in long term live cell imaging experiments.

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