Journal
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Volume 644, Issue -, Pages 93-99Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2018.01.008
Keywords
Antimicrobial; Anticancer; Oral cancer; Polyphyllin G; Cell cycle arrest; Metastasis
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Plant natural products have long been considered to be important sources of bioactive molecules. A large number of antimicrobial and anticancer agents have been isolated form plants. In the present study we evaluated the antimicrobial and anticancer activity of a plant derived secondery metabolite, Polyphyllin G. The results of antibacterial assays showed that Polyphyllin G prevented the growth of both Gram-positive and Gram-negative bacteria with minimum inhibitory concentrations (MICs) ranging from 13.1 to 78 mu g/ml. Antifungal activity measured as inhibition of mycelium growth ranged between 38.32 and 56.50%. Further Polyphyllin G was also evaluated against a panel of cancer cell lines. The IC50 of Polyphyllin G ranged from 10 to 65 mu M. However the IC50 of Polyphyllin G was found to be comparatively high (120 mu M) against the normal FR2 cancer cell line. The lowest IC50 of 10 mu M was found against the oral cancer cell line OECM-1. Therefore further studies were carried out on this cell line only. Our results indicated that Polyphyllin G induced cell arrest in oral cancer OECM-1 cells by inactivation of cdc25C-cdc22 via ATM-Chk 1/2 stimulation. Therefore, we propose that Polyphyllin G might prove a lead molecule in the management of oral cancers and at the same time may prevent the growth of opportunistic microbes.
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