Journal
AQUATIC TOXICOLOGY
Volume 195, Issue -, Pages 1-7Publisher
ELSEVIER
DOI: 10.1016/j.aquatox.2017.11.020
Keywords
Scylla paramamosain; Cadmium; Histopathological change; Stress response genes
Categories
Funding
- Zhejiang Provincial Key Project of Science and Technology Research [2015C02054]
- Zhejiang Provincial Key Project of Aquaculture New Varieties Breeding [2016C02055-8]
- China Postdoctoral Science Foundation [2016M601949]
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Cadmium (Cd) is a heavy metal that accumulates easily in organisms and causes several detrimental effects, including tissue damage. Cd contamination from anthropogenic terrestrial sources flows into rivers, and through estuaries to the ocean. To evaluate the toxic effects of Cd on estuary crustaceans, we exposed the mud crab Scylla paramamosain to various Cd concentrations (0, 10.0, 20.0, and 40.0 mg/L) for 24 h. We also exposed mud crabs to a fixed Cd concentration (20.0 mg/L) for various periods of time (0, 6, 12, 24, 48, and 72 h). We observed that after exposure to Cd, the surfaces of the gill lamellae were wrinkled, and the morphologies of the nuclei and mitochondria in the hepatopancreas were altered. We analyzed the expression profiles of 36 stress-related genes after Cd exposure, including those encoding metallothioneins, heat shock proteins, apoptosis-related proteins, and antioxidant proteins, with quantitative reverse transcription PCR. We found that exposure to Cd altered gene expression, and that some genes might be suitable bioindicators of Cd stress. Gene expression profiles were organ-, duration-, and concentration-dependent, suggesting that stress-response genes might be involved in an innate defense system for handling heavy metal exposure. To the best of our knowledge, this study is the first one of histopathology and stress-response gene expression pattern of Scylla paramamosain after Cd exposure. Our work could increase our understanding of the effect of environmental toxins on estuary crustaceans.
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