Effect of some safe feed additives on growth performance, blood biochemistry, and bioaccumulation of aflatoxin residues of Nile tilapia fed aflatoxin-B-1 contaminated diet

This work was performed to evaluate the effects of dietary supplementation with natural clay, coumarin, curcumin, vitamin C, probiotics and prebiotics on alleviating the harmful impacts resulting from aflatoxin B-1 (AFB(1)) toxicity in Nile tilapia. One hundred and sixty tilapia fish with average weight 7.360 +/- 0.048 were distributed into 8 groups. The first group fed the basal diet (T-1), the second group fed T-1 diet contaminated with 2 ppm AFB(1) (T-2) and the other groups fed the T-2 diet plus 20 g bentonite/kg diet (T-3), 5 g coumarin/kg diet (T-4), 5 g curcumin/kg diet (T-5), 100 mg vitamin C/kg diet (T-6), 1 g Bactocell (R)/kg diet (T-7) or 3 g Bio-Mos (R)/kg diet (T-8) for 14 weeks. Fish fed T-2 diet had reduced final body weight, weight gain and feed conversion by 19.85, 24.18 and 34.21%, respectively compared with fish fed T-1 diet. The T-6 group recorded the highest growth rate (25.20%) followed by T-5 (23.62%) and (T-3) compared with the T-2 group. Also, T-8 and T-3 groups recorded the best feed conversion ratio. Blood hemoglobin, total erythrocyte, total protein, albumin, and globulin concentrations decreased with aflatoxin contamination in T-2 group, while leukocytes enumerate increased. Supplemented aflatoxin contaminated diets with tested feed additives increased the concentration of hemoglobin, total erythrocyte, total protein, albumin, and globulin compared with the T-2 group, while the leukocytes decreased. Aflatoxin residues increased in fish fed T-2 diet compared to the other fish groups. Supplemented aflatoxin contaminated diets with tested feed additives decreased the level of aflatoxin residues.