Journal
EXTREMOPHILES
Volume 19, Issue 2, Pages 525-537Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s00792-015-0738-1
Keywords
Culturable haloarchaea; Genus-level diversity; Haloferax spp.; Meromictic lake; Site-specific diversity
Categories
Funding
- Romanian National Authority for Scientific Research, CNCS-UEFIS-CDI [PN-II-ID-PCE-2011-3-0546]
- Romanian Ministry of National Education [PN 09-360201]
- Institute of Biology Bucharest of Romanian Academy [RO1567-IBB05/2014]
- [POSDRU/159/1.5/S/132400]
Ask authors/readers for more resources
Perennially stratified salt lakes situated in the Transylvanian Basin (Central Romania) were surveyed for the diversity of culturable halophilic archaea (Fam. Halobacteriaceae). The physical and chemical characteristics of the waters indicated that all the investigated lakes were meromictic and neutral hypersaline. Samples collected from upper, intermediate, and deeper water layers and sediments were used for the isolation of halophilic strains followed by 16S rRNA gene-based identification and phenotypic characterization. The phylogenetic analysis of the 16S rRNA gene sequences revealed that all 191 isolates reported in this study and 43 strains previously isolated were affiliated with the family Halobacteriaceae and classified to 18 genera. Haloferax was the most frequently isolated genus (similar to 47 %), followed by Halobacterium spp. (similar to 12 %), and Halorubrum spp. (similar to 11 %). Highest culturable diversity was detected in BrA cent ncoveanu Lake, the oldest and saltiest of all studied lakes, while the opposite was observed in the most stable and least human-impacted Fr Fund Lake. One strain from Ursu Lake might possibly constitute a novel Halorubrum species as shown by phylogenetic analysis. Several haloarchaeal taxa recently described in Asian (i.e., Iran, China) saline systems were also identified as inhabiting the Transylvanian salt lakes thus expanding our knowledege on the geographic distribution of Halobacteriaceae.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available