4.7 Article

Metabolic engineering of Clostridium tyrobutyricum for enhanced butyric acid production with high butyrate/acetate ratio

Journal

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 102, Issue 10, Pages 4511-4522

Publisher

SPRINGER
DOI: 10.1007/s00253-018-8954-0

Keywords

Metabolic engineering; Butyric acid; Clostridium tyrobutyricum; Butyrate/acetate ratio; Butyryl-CoA/acetate CoA transferase; Crotonase

Funding

  1. National Natural Science Foundation of China [21676098]
  2. State Key Laboratory of Pulp and Paper Engineering [2017C03]
  3. China Postdoctoral Science Foundation [2017M612667]
  4. Fundamental Research Funds for the Central Universities [2017BQ084]

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Butyric acid fermentation by Clostridium couples with the synthesis of acetic acid. But the presence of acetic acid reduces butyric acid yield and increases separation and purification costs of butyric acid. Hence, enhancing the butyrate/acetate ratio is important for economical butyric acid production. This study indicated that enhancing the acetyl-CoA to butyrate flux by overexpression of both the butyryl-CoA/acetate CoA transferase (cat1) and crotonase (crt) genes in C. tyrobutyricum could significantly reduce acetic acid concentration. Fed-batch fermentation of ATCC 25755/cat1 + crt resulted in increased butyrate/acetate ratio of 15.76 g/g, which was 2.24-fold higher than that of the wild-type strain. Furthermore, in order to simultaneously increase the butyrate/acetate ratio, butyric acid concentration and productivity, the recombinant strain ATCC 25755/ppcc (co-expression of 6-phosphofructokinase (pfkA) gene, pyruvate kinase (pykA) gene, cat1, and crt) was constructed. Consequently, ATCC 25755/ppcc produced more butyric acid (46.8 vs. 35.0 g/L) with a higher productivity (0.83 vs. 0.49 g/L.h) and butyrate/acetate ratio (13.22 vs. 7.22 g/g) as compared with the wild-type strain in batch fermentation using high glucose concentration (120 g/L). This study demonstrates that enhancing the acetyl-CoA to butyrate flux is an effective way to reduce acetic acid production and increase butyrate/acetate ratio.

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