4.3 Article

TASOS1 AND TATM20 GENES EXPRESSION AND NUTRIENT UPTAKE IN WHEAT SEEDLINGS MAY BE ALTERED VIA EXCESS CADMIUM EXPOSURE AND INOCULATION WITH AZOSPIRILLUM BRASILENSE SP7 UNDER SALINE CONDITION

Journal

APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH
Volume 16, Issue 2, Pages 1797-1817

Publisher

CORVINUS UNIV BUDAPEST
DOI: 10.15666/aeer/1602_17971817

Keywords

chlorophyll; carotenoid; Fe; Na; K; dry weight

Funding

  1. University of Isfahan

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Excess of salt and cadmium (Cd) damage the plant's growth; however, Cd toxicity is more severe under saline condition. Triticum aestivum transmembrane 20 (TaTM20) and salt overly sensitive (SOS) respond to salinity. Moreover, wheat-Azospirillum association system leads to an increase in wheat tolerance to abiotic stresses. Less information exists related to the effect of salinity and Cd under wheat-Azospirillum associated system. Therefore, this experiment was conducted to evaluate the effect of Azospirillum-wheat association under salinity and/or Cd stresses. Wheat seedlings (Sardari cultivar) inoculated with Azospirillum brasilense Sp7and grown for five days, and then transferred into hydroponic media for five more days with and without 200 mmol NaCl and/or 50 mg L-1 Cd, respectively. Root and shoot samples were separated and then dry weight, proline, photosynthetic pigments, catalase (CAT) and ascorbate peroxidase (APX) activities, Cd, Fe, Ca, Na, K were measured. Simultaneously, relative expression of TaSOS1 and TaTM20 in the root were measured. The results show salinity and/or Cd have increased root's TaSOS1 expression and higher upregulation was seen in inoculated seedlings. Meanwhile TaTM20 gene expression upregulated only under Cd and Cd plus salinity conditions. Salinity and/or Cd were increased root and shoot proline, CAT, APX, Na+ and Cd whereas dry weight, pigments, Fe, Ca, K were decreased. A. brasilense could improve salinity and Cd adverse effects by more upregulation of TaSOS1 transcript level, lower Na/K ratio and less Fe and Ca deficiency, higher pigments, proline and APX and CAT activities to produce more dry weight.

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