Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 186, Issue 4, Pages 1061-1073Publisher
SPRINGER
DOI: 10.1007/s12010-018-2799-y
Keywords
Zucchini; Enzymatic activity; Functionalization; Self-assembled monolayers (SAM); Nanobiosensor; Atomic force microscopy (AFM)
Funding
- Cnpq
- Capes
- Fapergs
- Finep
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A cantilever nanobiosensor functionalized with vegetable source of peroxidase was developed as an innovative way for glyphosate herbicide detection over a wide concentration range (0.01 to 10mgL(-1)) using atomic force microscopy (AFM) technique. The extract obtained from zucchini (Cucurbita pepo source of peroxidase), with high enzymatic activity and stability has been used as bio-recognition element to develop a nanobiosensor. The polarization-modulated reflection absorption infrared spectroscopy (PM-RAIRS) demonstrated the deposition of enzyme on cantilever surface using self-assembled monolayers (SAM) by the presence of the amide I and II bands. The detection mechanism of glyphosate was based on the changes in surface tension caused by the analyte adsorption, resulting in a conformational change in the enzyme structure. In this way, the results of nanobiosensor demonstrate the potential of the sensing device for detecting glyphosate with a detection limit of 0.028mgL(-1).
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