Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 186, Issue 4, Pages 816-833Publisher
SPRINGER
DOI: 10.1007/s12010-018-2775-6
Keywords
Thermophilic xylanase; Eupenicillium parvum; Characterization; Feruloylated oligosaccharides; Wheat bran
Funding
- This work was supported by grants from a 948 Research Project (No. 2013-4-16) from State Forestry Administration of China, the Natural Science Fund for Provincial Colleges and University of Jiangsu Province, China (15KJB220003), the Research Fund for the Advanced Talents, Nanjing Forestry University (GXL201311) and the Priority Academic Program Development of Jiangsu Higher Education Institutions. [(No. 2013-4-16)] Funding Source: Medline
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Two new endo-1,4-beta-xylanases encoding genes EpXyn1 and EpXyn3 were isolated from mesophilic fungus Eupenicillium parvum 4-14. Based on analysis of catalytic domain and phylogenetic trees, the xylanases EpXYN1 (404 aa) and EpXYN3 (220 aa) belong to glycoside hydrolase (GH) family 10 and 11, respectively. Both EpXYN1 and EpXYN3 were successfully expressed in Pichia pastoris and the recombinant enzymes were characterized using beechwood xylan, birchwood xylan, or oat spelt xylan as substrates, respectively. The optimum temperatures and pH values were 75 degrees C and 5.5 for EpXYN1, and 55 degrees C and 5.0 for EpXYN3. EpXYN1 exhibited a high stability at high temperature (65 degrees C) or at pH values from 8 to 10. EpXYN3 kept over 80% enzymatic activity after treatment at pH values from 3 to 10. The specific activities of EpXYN1 and EpXYN3 were 384.42 and 214.20U/mgusing beechwood xylan as substrate, respectively. EpXYN1 showed lower K-m values and higher specific activities toward different xylans compared to EpXYN3. Thin-layer chromatography analysis indicated that the hydrolysis profiles of xylans or xylo-oligosacharides were different by EpXYN1and EpXYN3. EpXYN3 had a higher efficiency than EpXYN1 in production of feruloylated oligosaccharides (FOs) from de-starched wheat bran. The maximum levels of FOs released by EpXYN1 and EpXYN3 were 11.1 and 14.4mol/g, respectively. In conclusion, the two xylanases are potential candidates for various industrial applications.
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