Antioxidant, cytotoxic and antimicrobial activities of green synthesized silver nanoparticles from crude extract of Bergenia ciliata

Background: The methods like bio-green are advantageous over chemical and physical methods due to ecofriendly and cost-effective synthesis of nanoparticles. Current study was designed for green synthesis of silver nanoparticles (AgNPs) and their biological evaluation. Methods: Methanolic extract of Bergenia ciliata (BC) rhizomes prepared by maceration was used for the synthesis of AgNPs and confirmed by UV-visible and Fourier Transform Infra-Red (FTIR) spectroscopy. Further field emission scanning electron microscope (SEM) was used for the shape and size determination. In vitro Antioxidant, antimicrobial and cytotoxic potential was determined by using standard protocols. Results: The nanoparticles were spherical in shape having average particle size of 35 nm. FTIR analysis revealed the possible involvement of phyto-constituents in silver nanoparticles of crude extract. Green synthesized nanoparticles (BCAgNPs) showed the enhanced antioxidant properties compared to the crude extract. These nanoparticles showed the cytotoxic effects against brine shrimp (Artemia salina) nauplii with a value of 33.92 mu g/ml LD50. BCAgNPs were found effective against the pathogenic fungal and bacterial strains in comparison to the Bergenia ciliata extract. Conclusion: Green synthesized BCAgNPs showed enhanced biological activities. Present results also support the advantages of using bio-green method for the production of nanoparticles having the potential of antimicrobial and cytotoxic activities. (C) 2016 Future University. Production and hosting by Elsevier B.V.