Paenibacillus bryophyllum sp. nov., a nitrogen-fixing species isolated from Bryophyllum pinnatum

A nitrogen-fixing, endospore-forming bacterium, designated strain L201(T) was isolated from the leaves of Bryophyllum pinnatum growing in South China Agricultural University. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain L201(T) is affiliated with the genus Paenibacillus, and closely related to Paenibacillus albidus Q4-3(T) (97.4%), Paenibacillus odorifer DSM 15391(T) (97.3%) and Paenibacillus borealis DSM 13188(T) (97.2%). The main fatty acids components was anteiso-C-15:0 (48.1%). The predominant isoprenoid quinone was MK-7. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The G+C content of strain L201(T) was 43.9%. DNA-DNA relatedness between L201(T) and the reference strain was 29.8%. Biological and biochemical tests, protein patterns, genomic DNA fingerprinting and comparison of cellular fatty acids distinguished strain L201(T) from the closely related Paenibacillus species. Based on these data, the novel species Paenibacillus bryophyllum sp. nov. is proposed, with the type strain L201(T)(=KCTC 33951 (T)=GDMCC 1.1251 (T)).