Ancylomarina psychrotolerans sp nov., isolated from sediments of Fildes Peninsula and emended the description of genus Ancylomarina

A Gram-stain negative, obligately anaerobic, non-motile, asporogenous long rod-shaped and non-flagellated bacterial strain, designated 4SWWS2-6(T), was isolated from sediment in the intertidal zone of Fildes Peninsula, Antarctica. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 4SWWS2-6(T) belongs to the genus Ancylomarina and showed high sequence similarity with Ancylomarina subtilis FA102(T) (96.5%). Optimal growth occurred at pH 6.5, 16 A degrees C and in the presence of 3% (w/v) NaCl. Strain 4SWWS2-6(T) contained menaquinone-7 (MK-7) as the major respiratory quinone and held iso-C-15:0, anteiso-C-15:0 and iso-C-15:0 3-OH as the major cellular fatty acids. The major polar lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, an aminolipid, two unidentified lipids and an unidentified phospholipid. The DNA G + C content of strain 4SWWS2-6(T) was 37.6 mol%. On the basis of the polyphasic analyses, strain 4SWWS2-6(T) is considered to represent a novel species in the genus Ancylomarina, for which the name Ancylomarina psychrotolerans sp. nov. is proposed. The type strain is 4SWWS2-6(T) (= KCTC 15504(T) = MCCC 1K01618(T)).