Journal
BIOMEDICAL PHYSICS & ENGINEERING EXPRESS
Volume 2, Issue 3, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/2057-1976/2/3/035001
Keywords
3D neural cell culture; scaffold; two-photon polymerization; Ca2+ imaging; dissociated hippocampal cultures
Funding
- Russian Science Foundation [14-25-00055]
- Ministry of Education and Science of the Russian Federation [02.B.49.21.0003]
- Lobachevsky State University of Nizhni Novgorod [02.B.49.21.0003]
- DFG Excellence Cluster REBIRTH
- German Low Saxony project Biofabrication for NIFE
- Russian Science Foundation [14-25-00055] Funding Source: Russian Science Foundation
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Zr-Si organic-inorganic scaffolds fabricated by a two-photon polymerization technique were used for the primary culture of mouse embryonic neural cells. Weobserved that dissociated hippocampal cells adhere to the scaffolds, produce neurites, elongate and differentiate into adult neurons. Neuronal outgrowth and synaptogenesis were confirmed by immunohistochemical staining with antibodies against beta III-Tubulin and synaptophysin. The formation of a functional neural network was assessed by the measurement of spontaneous activity using Ca2+ imaging of dissociated hippocampal cultures grown on Zr-Si scaffolds. The results of this study suggest that two-photon-induced polymerization of organic-inorganic hybrid biomaterials provides a robust model for 3D neuronal tissue engineering studies.
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