Journal
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
Volume 18, Issue 8, Pages 1131-1137Publisher
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1871520618666180412114352
Keywords
Curcumin; chitosan nanoparticles; cervical cancer; cellular uptake; LDH assay; SiHa cells
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Funding
- Department of Science and Technology (SERB), New Delhi (India) [SB/YS/LS-296/2013]
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Background: Curcumin is a potent anticancer agent and has great potential efficacy against different types of cancers. A major disadvantage of curcumin, however, is its poor solubility and bioavailability. Objective: The aim of the present work is to synthesize ehitosan and curcumin-loaded chitosan nanoparticles and their characterization through various physicochemical methods and cellular uptake in cervical cancer cell line SiHa. Method: Chitosan nanoparticles were synthesized through the method of ionic gelation of chitosan with sodium Tripolyphosphate (TPP). In addition, the internal structure of chitosan nanoparticles and curcumin loaded chitosan nanoparticles were characterized by DLS, UV-Visible spectrophotometer, DSC, LCMS and LDH assay. Results: The studies presented demonstrate that curctunin-loaded chitosan nanoparticles showed increased uptake in the SiHa cells as compared to free curcumin and chitosan nanoparticles did not show any significant uptake in SiHa cell line. The curcumin-loaded chitosan nanoparticles released more lactate and lower ATP as compared to native curcumin in cervical cancer lines such as SiHa, CaSki and HcLa. Conclusion: Thus, chitosan based curcumin nanoparticles could be used as a potent vector / delivery agent for drug targeting in the treatment of cervical cancer.
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