Journal
BIOMEDICAL MATERIALS
Volume 11, Issue 1, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1748-6041/11/1/015019
Keywords
hydrogel encapsulation; 3D microwell; photo-crosslinkable hydrogel; embryonic stem cell; neuronal differentiation
Funding
- National Research Foundation (NRF) - Ministry of Science, ICT & Future Planning (MSIP) [2015R1A2A1A15054236]
- BioNano Health-Guard Research Center - MSIP of Korea as a Global Frontier Project, Korea [H-GUARD_2014M3A6B2060503]
- Leading Foreign Research Institute Recruitment Program through the NRF - MSIP [2013K1A4A3055268]
- National Research Foundation of Korea [2013K1A4A3055268, 2015R1A2A1A15054236, 2013M3A6B2078950] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
We developed a photo-crosslinkable hydrogel-encapsulated three-dimensional (3D) microwell array for studying embryonic stem (ES) cell-derived neuronal differentiation. ES cells were cultured for 5 d in microwells and were subsequently encapsulated by photo-crosslinkable gelatin methacrylate (GelMA) and polyethylene glycol (PEG) hydrogels for an additional 7 d. We observed that ES cells cultured in PEG microwells became uniform-sized embryoid bodies (EBs) compared to those in GelMA microwells. Although ES cells were encapsulated by photo-crosslinkable GelMA and PEG hydrogels, they were highly viable. We demonstrated that uniform-sized EBs encapsulated by GelMA hydrogels in PEG microwells are largely differentiated into neuronal cells. It was revealed that neurites at the periphery of EBs in PEG microwells largely extended into the interface between GelMA hydrogels and PEG microwells for generating neuronal networks. Therefore, this photo-crosslinkable GelMA hydrogel-encapsulated PEG microwell array could be a potentially powerful tool for neurodegenerative disease applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available