Visible Spectrophotometric Determination of Chlorpheniramine Maleate and Diphenhydramine Hydrochloride in Raw and Dosage form Using Potassium Permanganate

Two simple, rapid and sensitive spectrophotometric methods developed for Chlorpheniramine Maleate (CPM) and Diphenhydramine Hydrochloride (DPH) determination in pure and pharmaceutical preparation using Potassium Permanganate. The solvent system used was potassium permanganate. The method developed by adding a known amount of permanganate to CPM and DPH in acid and alkaline medium, the unreacted permanganate was determined at 550 nm; method A and bluish green colour of Manganate at 610 nm; method B. In method A decrease in absorbance or method B increase in absorbance as concentrations of CPM and DPH was measured. Beers law was obeyed at a range of 2.5 to 20 mu g / ml in both the methods A and B. The method was validated as per International Council for Harmonisation guideline. The proposed methods were effectively used for the determination of CPM and DPH in commercially available syrup. The average percentages of recoveries of CPM were 99.20 +/- 1.29% (method A), 100.6% +/- 1.43% (method B); DPH 98.50 +/- 1.29% (method A) and 100.20 +/- 1.43% (method B). The methods were efficiently validated and used for quantitative determination of Chlorpheniramine maleate and Diphenhydramine Hydrochloride in pure and syrup preparations.