Glyphosate affects swine ovarian and adipose stromal cell functions

Although Glyphosate (GLY) is a widely used pesticide, its effects on ovarian function and stem cell differentiation are still largely unknown. Therefore, as a contribution on this subject, the present work reports an investigation of the in vitro effects of GLY on swine granulosa cells and adipose stromal cells (ASCs). The effect of GLY at different doses (0.2, 4 and 16 mu g/mL) was evaluated on granulosa cells growth (BrDU incorporation and ATP production), steroidogenesis (17-beta estradiol and progesterone secretion) and redox status (superoxide and nitric oxide production and non-enzymatic scavenging activity). GLY has been shown to inhibit cell growth, 17-beta estradiol and non-enzymatic scavenging activity and to increase progesterone and nitric oxide secretion (P < 0.05). In addition, GLY significantly decreased the viability of ASCs (P < 0.001), and inhibited their adipogenic differentiation. These data indicate that GLY alters the main features of granulosa cells and ASCS thus suggesting that GLY could affect both reproductive function and adipose tissues homeostasis.