Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 57, Issue 38, Pages 12420-12424Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201806002
Keywords
DNA; mitochondria; phototherapy; ruthenium; signal peptide
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Funding
- Science Foundation Ireland [14/IA/2488, 17/TIDA/4930]
- Irish Research Council
- Science Foundation Ireland (SFI) [17/TIDA/4930] Funding Source: Science Foundation Ireland (SFI)
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Mitochondrial DNA (mtDNA) plays a crucial but incompletely understood role in cellular biochemistry and etiology of numerous disease states. Thus, there is an urgent need for targeted probes that can dynamically respond to changes to mtDNA such as copy number in live cells, but it is difficult to permeate the mitochondrial membrane of the living cell. Now, a ruthenium(II) light-switching probe targeted by peptide vectorization selectively to mitochondrial nucleoids is presented. Evidence for DNA binding by the probe in live cells is derived from confocal fluorescence microscopy, resonance Raman, and luminescence lifetime imaging. While viable under imaging conditions, specific staining of mitochondrial DNA permitted efficient and selective photoinduced toxicity on a cell-by-cell basis under higher excitation intensities. This powerful combination of imaging and photocytotoxicity is an important step towards realizing phototheranostic application of such Ru-II probes.
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