Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 57, Issue 34, Pages 10873-10878Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201804304
Keywords
ATP; brown adipocytes; fluorescent probes; live cell imaging; protein engineering
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Funding
- Japan Agency for Medical Research and Development (AMED) [JP17gm5810001, 25350970, 16K01922, 16H06044]
- JSPS Core-to-Core Program (Advanced Research Networks)
- Grants-in-Aid for Scientific Research [16K01922, 16H06044, 25350970] Funding Source: KAKEN
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Adenosine triphosphate (ATP) provides energy for the regulation of multiple cellular processes in living organisms. Capturing the spatiotemporal dynamics of ATP in single cells is fundamental to our understanding of the mechanisms underlying cellular energy metabolism. However, it has remained challenging to visualize the dynamics of ATP in and between distinct intracellular organelles and its interplay with other signaling molecules. Using single fluorescent proteins, multicolor ATP indicators were developed, enabling the simultaneous visualization of subcellular ATP dynamics in the cytoplasm and mitochondria of cells derived from mammals, plants, and worms. Furthermore, in combination with additional fluorescent indicators, the dynamic interplay of ATP, cAMP, and Ca2+ could be visualized in activated brown adipocyte. This set of indicator tools will facilitate future research into energy metabolism.
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