Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 57, Issue 35, Pages 11378-11383Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201807314
Keywords
dielectrophoresis; loop-mediated isothermal amplification; microfluidic devices; self-digitization; single-cell analysis
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Funding
- NIH [R21EB018831, UG3CA211139]
- NATIONAL CANCER INSTITUTE [UG3CA211139] Funding Source: NIH RePORTER
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The design and fabrication of a self-digitization dielectrophoretic (SD-DEP) chip with simple components for single-cell manipulation and downstream nucleic acid analysis is presented. The device employed the traditional DEP and insulator DEP to create the local electric field that is tailored to approximately the size of single cells, enabling highly efficient single-cell capture. The multistep procedures of cell manipulation, compartmentalization, lysis, and analysis were performed in the integrated microdevice, consuming minimal reagents, minimizing contamination, decreasing lysate dilution, and increasing assay sensitivity. The platform developed here could be a promising and powerful tool in single-cell research for precise medicine.
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