Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 57, Issue 29, Pages 8891-8895Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201802796
Keywords
cellular microviscosity; cellular polarity; fluorescent probes; mitochondria
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Funding
- Canadian Institutes of Health Research
- National Institutes of Health [R01GM116886]
- National Sciences and Engineering Research Council
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The measurement of physicochemical parameters in living cells can provide information on individual cellular organelles, helping us to understand subcellular function in health and disease. While organelle-specific chemical probes have allowed qualitative evaluation of microenvironmental variations, the simultaneous quantification of mitochondrial local microviscosity ((m)) and micropolarity (E-m), along with concurrent structural variations, has remained an unmet need. Herein, we describe a new multifunctional mitochondrial probe (MMP) for simultaneous monitoring of (m) and E-m by fluorescence lifetime and emission intensity recordings, respectively. The MMP enables highly precise measurements of (m) and E-m in the presence of a variety of agents perturbing cellular function, and the observed changes can also be correlated with alterations in mitochondrial network morphology and motility. This strategy represents a promising tool for the analysis of subtle changes in organellar structure.
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