Journal
ANALYTICAL METHODS
Volume 10, Issue 8, Pages 832-835Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7ay02755a
Keywords
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Funding
- Ministry of Science and Technology, Taiwan [96-2113-M-320-002, MOST 105-2113-M-303-001]
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A simple and rapid method for direct quantitation of drugs in human urine samples was developed using a system composed of an automatic column switch and two home-made capillary immunoaffinity columns (CIACs, 100 m x 15 cm). Before being injected by an autosampler into the system, the urine sample was filtered through a 0.22 m membrane. The alternating column regeneration was achieved by using two identical CIACs. One column underwent analyte elution while the other column underwent column regeneration simultaneously. 6-Acetylmorphine (6AM) and oxycodone (OCOD) were studied as examples of opiates in urine. Linear calibration curves were obtained in the concentration ranges of 40-200 ng mL(-1) and 50-400 ng mL(-1), with a limit of quantitation of 22 ng mL(-1) and 47 ng mL(-1) for 6AM and OCOD, respectively. The results of this method exhibited good inter-day accuracy and precision. Furthermore, the present method was successfully applied to the determination of 6AM in urine samples of drug abusers.
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