Journal
ANALYTICAL CHEMISTRY
Volume 90, Issue 12, Pages 7122-7126Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b01631
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Funding
- National Natural Science Foundation of China [21778001, 21372005]
- Anhui University
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We reported the first lysosome targeted two-photon fluorescent probe (Lyso-NP) as a viscosity probe for monitoring autophagy. The fluorescence lifetime of Lyso-NP exhibited an excellent linear relationship with viscosity value (R-2 = 0.99, x = 0.39). Lyso-NP also showed the specific capability for imaging lysosomal viscosity under two-photon excitation at 860 nm along with good biocompatibility. More importantly, Lyso-NP could be used to monitor the autophagy process in living cells by quantitatively detecting lysosomal viscosity changes during the membrane fusion process via two-photon fluorescence lifetime imaging.
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