Journal
ANALYTICAL CHEMISTRY
Volume 90, Issue 12, Pages 7415-7421Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b00853
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Funding
- National Key RD Program [2016YFD0500706]
- National Natural Science Foundation of China [21778020]
- Sci-tech Innovation Foundation of Huazhong Agriculture University [2662017PY042, 2662018PY024]
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The sensitive and accurate detection methods for PEDV antibody have practical significance for the prevention and treatment of PEDV. In this work, a new multiple pathways signal amplification method was proposed to construct a sensitive electrochemiluminescence (ECL) platform for the detection of PEDV antibody. Using Au NP-modified graphene nanosheet (Au-GN) as the substrate, antibody-antigen reaction as the recognition unit, rolling circle amplification (RCA) for signal enhancement, and assembled cascade Ru-DNA nanotags as signal label, the proposed platform behaved with good specificity and sensitivity. The binding system of biotin-streptavidin, RCA, and Ru(bpy)(3)(2+)-doped silica nanoparticles (Ru SNPs) showed remarkable amplification efficiency, low background signal, and little nonspecific adsorption. Moreover, the proposed ECL sensor exhibited good analytical performance for PEDV antibody with a wide linear range from 0.1 pg mL(-1) to 5000 pg mL(-1) with a detection limit of 0.05 pg mL(-1) (S/N = 3). The proposed strategy exhibited the advantages of excellent stability and sensitivity for determination of the PEDV antibody, which was easy to prepare and had a good application prospect.
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