Journal
ANALYTICAL CHEMISTRY
Volume 90, Issue 5, Pages 3009-3012Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b00222
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Funding
- AgriLife Research of Texas AM
- Governor's University Research Initiative (GURI) grant program of Texas A&M University, GURI Grant [12-2016]
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Rapid detection and identification of crop pathogens is essential for improving crop yield. Typical pathogen assaying methods, such as polymerase chain reaction (PCR) or enzyme-linked immunosorbent assay (ELISA), are time-consuming and destructive to the sample. Raman spectroscopy (RS) is a noninvasive nondestructive analytical technique that provides insight on the chemical structure of the specimen. In this study, we demonstrate that using a hand-held Raman spectrometer, in combination with chemometric analyses, we can distinguish between healthy and diseased maize (Zea mays) kernels, as well as between different diseases with 100% accuracy. Our analysis is portable and sample-agnostic, suggesting that it could be retooled for other crops and conducted autonomously.
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