Journal
ANALYTICAL CHEMISTRY
Volume 90, Issue 6, Pages 4221-4225Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b00576
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Funding
- Konya Food and Agriculture University (KFAU)
- Nanobiz RD Ltd.
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A novel ratiometric colorimetric and fluorescent dual probe based on Eriochrome Black T (EBT) Eu3+ complex was designed to detect dipicolinic acid (DPA), a major constituent of bacterial spores, with high sensitivity and selectivity. UV vis titration experiments demonstrated that EBT and Eu3+ ions formed a 1:1 coordination pair in water. In the presence of Eu3+ ions, the blue solution of EBT changed to magenta, however, upon the addition of DPA, the magenta color changed to blue immediately and characteristic fluorescence emission from DPA-Eu3+ complex was observed. In addition, the sensitivity of the system was further evaluated on Geobacillus stearothermophilus spores and as low as 2.5 X 10(5) spores were detected.
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