Journal
ANALYTICAL CHEMISTRY
Volume 90, Issue 7, Pages 4438-4444Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b04419
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Funding
- National Natural Science Foundation of China [21705047, 21335003]
- Chinese Post-Doctoral Fund [2017M621380]
- Fundamental Research Funds for the Central Universities [222201814030]
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Nitric oxide (NO) is a transcellular messenger involved in many physiological and pathological processes, but the real-time detection of NO in biological systems is still challenging due to its rapid diffusion, low concentration, and short half-life. A novel electrochemical sensing platform based on iron phthalocyanine (FePc) functionalized nitrogen-doped graphene (N-G) nanocomposites was constructed to achieve in situ monitoring of NO released from living cells on the sensing layer. By taking advantage of the synergetic effect of N-G and FePc nanocomposites, the N-G/FePc sensor displays excellent electrocatalytic activity toward NO with a high sensitivity of 0.21 mu A mu M-1 cm(-2) and a low detection limit of 180 nmol L-1. The following layer-by-layer assembly of poly-L-lysine (PLL) and Nafion further improved the capacity of resisting disturbance as well as the biocompatibility of the sensing interface. The flexible design of the ITO substrate based electrode provides a more controlled cellular biosensing system which could capture molecular signals immediately after NO released from human umbilical vein endothelial cells (HUVECs). The exhibited additional features of high sensitivity, rapid response, and ease of operation implies that the proposed N-G/FePc/Nafion/PLL ITO biosensor is a promising powerful platform in various complex biological systems.
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