Journal
ANALYTICAL CHEMISTRY
Volume 90, Issue 7, Pages 4478-4484Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b04798
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Funding
- Major State Basic Research Development Program [2013CB932702]
- National Natural Science Foundation of China [21275106, 21575095, 81502392, 3601317]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- 111 project
- Collaborative Innovation Center of Suzhou Nano Science and Technology
- 1000 Youth Talents plan of the Global Expert Recruitment Program
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As an important signaling molecule, hydrogen peroxide (H2O2) secreted externally by the cells influences cell migration, immunity generation, and cellular communications. Herein, we have developed a microfluidic approach with droplets in combination with Au nanoclusters for the sensitive detection of H2O2 secreted by a single cell. Isolated in the ultrasmall volume (4.2 nL) of a microdroplet, single-cell secreted H2O2 can initiate dramatic fluorescence changes of horseradish peroxidase-Au nanoclusters. We have demonstrated an ultrahigh sensitivity (200-400 attomole H2O2 directly measured from a single cell) with good specificity. It offers a useful research tool to study the cell-to-cell differences of H2O2 secretion at the single-cell level.
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