Journal
ANALYTICAL CHEMISTRY
Volume 90, Issue 5, Pages 3174-3182Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b04468
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Funding
- National Key Research and Development Program of China [2017YFD0400600]
- State Key Program of National Natural Science of China [U1608255]
- Natural Science Foundation of Liaoning Province [2015021015]
- Wellcome Trust Biomedical Resource [108430/Z/15/Z]
- Royal Society International Exchange [IE141171]
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Sialylated oligosaccharides are important components in mammalian milk. They play a key role in the health and growth of infants by helping to shape up infant's gastrointestinal microbiota and defense against infection by various pathogenic agents. A detailed knowledge of the structures, compositions, and quantities of the sialylated milk oligosaccharides (SMOs) is a prerequisite for understanding their biological roles. However, because of the presence of very large amounts of lactose and neutral oligosaccharides, accurate analysis of SMOs is difficult. A pretreatment step is required to remove lactose and neutral oligosaccharides but conventional off-line pretreatment methods are time-consuming and of poor reproducibility. In this presentation, we linked solid-phase extraction (SPE) with hydrophilic interaction chromatography (HILIC) followed by mass spectrometry (MS) identification for the analysis of SMOs. A SPE column with electrostatic repulsion function was used for removal of lactose and neutral oligosaccharides, a HILIC analytical column for separation of the SMOs, and negative-ion electrospray ionization tandem MS was used for their identification and sequencing. The success of the established online SPE-HILIC-MS method was demonstrated by profiling of SMOs in human to investigate detailed SMO changes during lactation period and in animals to compare the difference in SMO contents among the different species.
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