Journal
ANALYTICAL CHEMISTRY
Volume 90, Issue 4, Pages 2618-2624Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b04193
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Funding
- Centers for Disease Control and Prevention/National Institute for Occupational Safety and Health (CDC/NIOSH) [R010H011023-01A1]
- National Natural Science Foundation of China [21275063, 21575048]
- Science and Technology Development project of Jilin province, China [20150204010GX]
- National Postdoctoral Program for Innovative Talents [BX201700096]
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Carbon dots (CDs) combined with a nanomaterial-based quencher has created an innovative way for designing promising sensors. Herein, a novel fluorescent-sensing platform was designed for sensitive detection of organophosphorus pesticides (OPs). The preparation of CDs was based on one-step hydrothermal reaction of 3-aminobenzeneboronic acid. The fluorescence of CDs can be quenched by manganese dioxide (MnO2) nanosheets via the Forster resonance energy transfer (FRET). In the presence of butyrylcholinesterase (BChE) and acetylthiocholine, the enzymatic hydrolysate (thiocholine) can efficiently trigger the decomposition of MnO2 nanosheets, resulting in the recovery of CDs fluorescence. OPs as inhibitors for BChE activity can prevent the generation of thiocholine and decomposition of MnO2 nanosheets, accompanying the fluorescence turn-off of the system. So the BChE-ATCh-MnO2-CDs system can be utilized to detect OPs quantitatively based on the fluorescence turn on-off. Under the optimum conditions, the present FRET-based approach can detect paraoxon ranging from 0.05 to 5 ng mL(-1) with a detection limit of 0.015 ng mL(-1). Meanwhile, the present strategy also showed a visual color change in a concentration-dependent manner. Thus, the proposed assay can potentially be a candidate for OPs detection.
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