Journal
ANALYTICAL CHEMISTRY
Volume 90, Issue 7, Pages 4725-4732Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b05344
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Funding
- Natural Science Foundation of China [81671784, 21505027, U1301214]
- Beijing Nova Program [Z181100006218017]
- Youth Innovation Promotion Association of the Chinese Academy of Sciences [2018047]
- Guangdong and Guangzhou Planned Program in Science and Technology [2016201604030004, 2017B020207010, 201803020026]
- National Key Research and Development Program of China [SQ2017YFC160089]
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In this work, we outline a signal amplification strategy using the coordination chemistry between Fe(3+)and poly(glutamic acid) (PGA) for biosensing applications. The theoretical calculation based on density functional theory shows that PGA has a much higher binding affinity with Fe3+ than the other metal ions. Guided by this rationale, we prepare a PGA-mediated signal probe through conjugating PGA onto polystyrene (PS) nanoparticles to form a brushlike nanostructure for Fe3+ coordination. This PGA-PS brush (PPB) has a large loading capacity of Fe3+ with a number of 1.92 x 10(8) Fe atoms per nanoparticle that greatly amplifies the signals for assays in an enzyme-free way. Combined with ferrozine coloration-based readout, this PPB-mediated amplification is further applied for the enzyme-free immunoassay that shows an ultrahigh sensitivity for detection of microcystins-LR (12 pg/mL), a 5-fold enhancement compared with that of traditional enzyme-linked immunosorbent assay (ELISA) (60 pg/mL). In addition, the good stability, rapid response, and long shelf life make this enzyme-free amplification strategy a promising platform for point-of-care biosensing applications.
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