Journal
ANALYTICAL CHEMISTRY
Volume 90, Issue 3, Pages 1511-1515Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b04950
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Funding
- ImPACT Program of the Council for Science, Technology and Innovation, Cabinet Office, the Government of Japan
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Bioinspired pore sensing for selective detection of flagellated bacteria was investigated. The Au micropore wall surface was modified with a synthetic peptide designed from toll-like receptor 5 (TLR5) to mimic the pathogen-recognition capability. We found that intermolecular interactions between the TLRS-derived recognition peptides and flagella induce ligand-specific perturbations in the translocation dynamics of Escherichia coli, which facilitated the discrimination between the wild-type and flagellin-deletion mutant(Delta fliC) by the resistive pulse patterns thereby demonstrating the sensing of bacteria at a single-cell level. These results provide a novel concept of utilizing weak intermolecular interactions as a recognition probes for single-cell microbial identification.
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