Journal
ANALYTICAL CHEMISTRY
Volume 90, Issue 4, Pages 2716-2724Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b04684
Keywords
-
Categories
Funding
- National Key Research and Development Program of China [2017YFC1601700, 2016YFE0106000]
- Natural Science Foundation of China [31701703, 31601555, U1301214, S2013030013338]
- Natural Science Foundation of Guangdong [31701703, 31601555, U1301214, S2013030013338]
- Guangdong Planned Program in Science and Technology [2016201604030004, 2017B020207010]
- Guangzhou Planned Program in Science and Technology [2016201604030004, 2017B020207010]
- Russian Science Foundation [14-16-00149]
- Russian Science Foundation [17-16-00012] Funding Source: Russian Science Foundation
Ask authors/readers for more resources
Immunoassay methods are important for monitoring beta-agonists illegally used for reducing animal fat deposition in livestock. However, there is no simultaneous screening surveillance immunoassay for detecting various beta-agonist chemicals that are possibly present in food. In this study, through the use of an R-(-)-salbutamol derivative as the immunizing hapten, an antibody recognizing 31 beta-agonists and analogues was generated for the first time. Three-dimensional quantitative structure-activity relationship (3D QSAR) revealed that strong steric and hydrophobic fields around the hapten spacer near C-2, as well as a chirality at C-1', dominantly modulated the class specificity of the raised antibody. However, a hapten spacer linked at C-2' or C-1 would lead to a narrow specificity, and the spacer charge at C-6 could affect the raised antibody specificity spectrum. A class specificity competitive indirect enzyme-linked immunosorbent assay (ciELISA) was established with an ideal recovery ranging from 81.8 to 118.3% based on the obtained antibody. With a good agreement to the HPLC/MS method, the proposed ciELISA was confirmed to be reliable for the rapid surveillance screening assay of beta-agonists in urine. This investigation will contribute to the rational design and control of the immunoassay specificity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available