Journal
MOLECULAR AND CLINICAL ONCOLOGY
Volume 5, Issue 1, Pages 107-110Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/mco.2016.889
Keywords
nm23-H1 gene; mutation; metastasis; colorectal adenocarcinoma
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Funding
- Shanghai Pudong Science and Technology Commission of China [PKJ2014-Y23]
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Nm23-H1 is a metastasis suppressor gene, which is has a reduced expression in patients with digestive system cancer. However, the mechanistic basis for the genetic instability remains unknown. To study the expression of the nm23-H1 gene in patients with colorectal cancer, polymerase chain reaction-single strand conformation polymorphism was used to analyze any point mutation, and immunohistochemistry was used to detect the expression of nm23-H1. Results revealed that all 63 specimens of Chinese human colorectal cancer tissues exhibit no point mutation. Among those 63 specimens, 19 (30%) exhibited positive immunostaining for the nm23-H1 protein and 44 (70%) exhibited negative immunostaining. These observations suggested that the protein and gene expression levels of nm23-H1 are reduced in colorectal cancer compared with the adjacent normal tissues, and the point mutation in the nm23-H1 gene is not the dominant cause of metastatic colorectal cancer.
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