Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 410, Issue 21, Pages 5165-5172Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-018-1170-z
Keywords
Quantum dots; Molecular imprinting; Clopyralid; Enzymatic amplification; Electrochemiluminescence
Funding
- National Natural Science Foundation of China [21765006, 21375031]
- Natural Science Foundation of Guangxi Province of China [2015GXNSFFA139005]
- High Level Innovation Team of Guangxi Colleges Universities
- High Level Innovation Team of Outstanding Scholars Program [Guijiaoren[2014]49]
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A new molecularly imprinted polymer electrochemiluminescence (ECL) sensor was developed for the detection of clopyralid (CPD) based on enzyme-biocatalyzed amplification. CdTe quantum dots were immobilized on the surface of an electrode by reaction with p-aminothiophenol preadsorbed on the electrode. Then a molecularly imprinted film was formed by electrochemical polymerization of o-phenylenediamine in the presence of CPD on the CdTe-modified gold electrode. During the analytical cycle, horseradish peroxidase (HRP)-labeled CPD was replaced by CPD in the sample. The amount of HRP on the molecularly imprinted polymer electrode decreased, and then less H2O2 was catalytically decomposed. Subsequently, the ECL intensity of the CdTe-H2O2 system was enhanced. There was a good linear relationship between ECL intensity and the concentration of CPD in the range from 2.0 x 10(-11) to 2.5 x 10(-10) mol/L and in the range from 2.5 x 10(-10) to 3.5 x 10(-8) mol/L. The detection limit was 4.1 x 10(-12) mol/L. The sensor was applied to determine CPD in vegetable samples.
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