Journal
SCIENCE IMMUNOLOGY
Volume 1, Issue 1, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciimmunol.aaf6628
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Funding
- U.S. NIH [AI048115, AI049342, AI090902, AI090742, AI097629, AI117703]
- NIH [AR48311, AI027767]
- Department of Pathology, UAB
- UAB Dart program [NIDCR-1T90DE022736]
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Developmental checkpoints eliminate B cells that synthesize defective immunoglobulin (Ig) heavy (HC) and light (LC) chains. The first checkpoint tests mu HCs paired with VpreB/lambda 5 in a pre-B cell receptor (pre-BCR) to determine whether the mu HCs will be able to bind conventional LCs to form membrane IgM. VpreB and lambda 5 also create a sensing site that interacts with the mu HCs antigen-binding region complementarity-determining region (CDR)-H3; however, whether this site contributes to Ig repertoire selection and function is unknown. We analyzed the amino acid content of CDR-H3s from HCs cloned from living and apoptotic pre-B cells and from IgG-antigen structures. Using a panel of D-H gene-targeted mice, we showed that progressively reducing CDR-H3 tyrosine content increasingly impaired pre-BCR checkpoint passage. Counting from cysteine at framework 3 position 96, we found that VpreB particularly selected for tyrosine at CDR-H3 position 101 and that Y101 also bound antigen in IgG-antigen structures. Therefore, in addition to its stabilization role in the pre-BCR, VpreB also acts as an early invariant antigen by selecting for particular CDR-H3 amino acids. These interactions shape the specificity of the IgG humoral response and may thus impose limitations on development of certain neutralizing antibodies.
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