Journal
ANALYTICA CHIMICA ACTA
Volume 1026, Issue -, Pages 133-139Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2018.04.014
Keywords
Papain@AuNCs; Fluorescence turn on; D-penicillamine; Metabolic process
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Funding
- National Natural Science Foundation of China [21575144, 91732103, 21727809, 21635008, 21621062]
- Chinese Academy of Sciences [QYZDJ-SSW-SLH034]
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Papain-stabilized gold nanoclusters (papain@AuNCs) with a red fluorescence emission at 639 nm have been generated successfully in aqueous solution. The fluorescence of papain@AuNCs could be quenched in the addition of Cu2+. Subsequently, a unique fluorescent probe based on papain@AuNCs-Cu2+ complex has been constructed for sensitive, selective and turn-on detection of D-penicillamine (D-pen). The sensing probe has exhibited a remarkable fluorescence enhancement in the presence of D-pen, which can be measured ranging from 30.0 mu M to 2.0 mM (r(2) = 0.991) with the detection limit of 5.0 mu M. Furthermore, the developed assay has been utilized to explore the metabolic process of D-pen in rats after intraperitoneal injection. As far as we concerned, the present study has reported the first analytical method for sensing serum D-pen in real time by using the fluorescent papain@AuNCs-Cu2+ complex. Our strategy paves a way for digging up the biological and clinical application of the fluorescent AuNCs. (C) 2018 Elsevier B.V. All rights reserved.
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