Magnetic framework composite as sorbent for magnetic solid phase extraction coupled with high performance liquid chromatography for simultaneous extraction and determination of tricyclic antidepressants

In this study, magnetic framework composites (MFCs) (Fe3O4@TMU-10) microspheres were successfully fabricated and applied as an effective sorbent for preconcentration of the two model tricyclic antidepressants (TCAs) amitriptyline and imipramine from biological samples. MFCs were fabricated by a step-by-step assembly, novel, simple and efficient strategy. The shell thickness of the Metal-organic frameworks (MOFs) could also be easily controlled by tuning the number of assembly cycles. By coupling magnetic solid-phase extraction (MSPE) with high-performance liquid chromatography with UV detector (HPLC-UV), a simple, reliable, fast, sensitive and cost-effective method for simultaneous determination of TCAs was developed. Under optimal conditions, the preconcentration factors and relative recoveries of the studied compounds were obtained in the range of 43-50 and 90.5-99.0% respectively. The calibration curves were obtained in the range of 5-800 mu g L-1 with reasonable linearity (R-2 > 0.9904) and the limits of detection (LODs) ranged between 2 and 4 mu g L-1 (based on S/N = 3). The relative standard deviations of intra- and inter-day tests ranged from 3.1 to 4.6% and from 4.3 to 5.2%, respectively. The results demonstrate that Fe3O4@TMU-10 core-shell magnetic microspheres combine advantages of MOFs and magnetic nanoparticles, and are the promising sorbents for rapid and efficient extraction of target analytes from urine and plasma complex biological samples. (C) 2018 Elsevier B.V. All rights reserved.