Journal
ANALYTICA CHIMICA ACTA
Volume 1033, Issue -, Pages 137-147Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2018.05.037
Keywords
Microfluidics; Aptamer; The attenuated total reflection surface-enhanced infrared absorption spectroscopy; Mercury ion
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Funding
- Ministry of Science and Technology, R.O.C. [MOST 104-2221-E-006-154-MY3, MOST 103-2221-E-006-093-MY3]
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A sensing platform based on the attenuated total reflection surface enhanced infrared absorption spectroscopy (ATR-SEIRAS) technique and immobilized aptamer has been proposed herein for the selective detection of mercury ions (Hg2+). In the proposed platform, 5'-thiolated 32-mer DNA probes with methylene blue at the 3 end were immobilized on a thin gold (Au) surface layer. Following Hg2+ ions interacting with T bases of the aptamer, T-Hg-T bonds are formed; resulting in a hairpin-shaped formation of the DNA and a detectable change in the IR absorbance of the sensing interface. Notably, the background noise produced by external molecules (e.g., water, non-specific binding molecules and bulk solution) is reduced to a negligible level by means of the ATR detection mode. It is shown that the proposed sensor has a linear response (R-2 = 0.986) with high sensitivity and good selectivity over the Hg2+ range of 0.01 mu M-50 mu M. (C) 2018 Elsevier B.V. All rights reserved.
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