Journal
ANALYTICA CHIMICA ACTA
Volume 1024, Issue -, Pages 84-92Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2018.04.030
Keywords
Glycopeptidome; Hydrophilic probe; Magnetic mesoporous materials; Endogenous glycopeptides enrichment; Saliva bio-samples
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Funding
- National Natural Science Foundation of China [21425518, 21405022, 21675034]
- National Basic Research Priorities Program of China [2013CB911201]
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As one of the most important post-translational modifications (PTMs) of peptidome, glycopeptidome is closely related to serious disease, especially to cancer. In order to specifically discover and analyze glycopeptidome biomarkers for clinical diagnosis of cancer on early-stage, it is crucial to develop efficient technique to analyze low-abundance of endogenous glycopeptides in biological samples. In this report, a hydrophilic probe in mesoporous pore (denoted as Fe3O4@mSiO(2)@G6P) was designed and prepared. By taking advantage of the excellent hydrophilicity and size-exclusion ability, we applied Fe3O4@mSiO(2)@G6P to capture glycopeptides from both horseradish peroxidase (HRP) and immunoglobulin (IgG) digests successfully. Moreover, a total of 39 and 25 endogenous glycopeptides were identified from healthy saliva and gastric saliva, respectively, indicating the great potential of this probe for the exploration of glycopeptidome biomarkers. (C) 2018 Elsevier B.V. All rights reserved.
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