Journal
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
Volume 7, Issue -, Pages 217-223Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bcab.2016.06.008
Keywords
Medium optimization; beta-carotene; Biomass; Dunaliella salina; Mixotrophic culture
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Funding
- Shiraz University of Medical Sciences, Shiraz, Iran [93-01-36-7749]
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Dunaliella salina is a well-known microalga with a high pharmaceutical and industrial value. It contains a high fatty acid, protein and beta-carotene content that makes it a fascinating choice for human food or feed. To improve the beta-carotene and biomass production and therefore its nutritional value in mixotrophic culture, a well-known commercial medium was optimized to the most favorable nutrient level using response surface methodology approach. The optimal growth medium conditions defined to be at a glucose concentration of 15.0 g L-1, a nitrate concentration of 1.80 g L-1, and a phosphate concentration of 0.013 g L-1. Suggested culture medium conditions allowed obtaining an 8.12 mg beta-carotene per gram dry weight (DW) and biomass concentration of 1.015 g L-1. The beta-carotene and biomass contents were increased 1.95 and 1.75 folds than their original values, respectively. (C) 2016 Elsevier Ltd. All rights reserved.
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