Journal
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
Volume 90, Issue 1, Pages 205-217Publisher
ACAD BRASILEIRA DE CIENCIAS
DOI: 10.1590/0001-3765201820140465
Keywords
soybean; carbohydrates; anti-nutritional factors; quantitative analysis; HPLC; NIR spectroscopy
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Funding
- Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) (Brazilian government)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) (Brazilian government)
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Soybeans contain about 30% carbohydrate, mainly consisting of non-starch polysaccharides (NSP) and oligosaccharides. NSP are not hydrolyzed in the gastrointestinal tract of monogastric animals. These NSP negatively affect the development of these animals, especially the soluble fraction. This work aimed to establish a method to quantify NSP in soybeans, using high performance liquid chromatography (HPLC), and to estimate correlations between NSP, oligosaccharides, protein and oil. Sucrose, raffinose + stachyose, soluble and insoluble NSP contents were determined by HPLC. Oil and protein contents were determined by near-infrared spectroscopy (NIRS). The soluble PNAs content showed no significant correlation with protein, oil, sucrose and raffinose + stachyose contents, but oligosaccharides showed a negative correlation with protein content. These findings open up the possibility of developing cultivars with low soluble NSP content, aiming to develop feed for monogastric animals.
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