Journal
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
Volume 90, Issue 1, Pages 631-644Publisher
ACAD BRASILEIRA DE CIENCIAS
DOI: 10.1590/0001-3765201720170453
Keywords
Langmuir-Blodgett; enzymes; biosensors; air-water interface
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Funding
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2015/10851-0]
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In this review we discuss about the immobilization of enzymes in Langmuir-Blodgett films in order to determine the catalytic properties of these biomacromolecules when adsorbed on solid supports. Usually, the conformation of enzymes depends on the environmental conditions imposed to them, including the chemical composition of the matrix, and the morphology and thickness of the film. In this review, we show an outline of manuscripts that report the immobilization of enzymes as LB films since the 1980's, and also some examples of how the surface properties of the floating monolayer prepared previously to the transfer to the solid support are important to determine the efficiency of the resulting device.
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