4.7 Article

Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses

Journal

EMERGING INFECTIOUS DISEASES
Volume 22, Issue 7, Pages 1295-1297

Publisher

CENTERS DISEASE CONTROL
DOI: 10.3201/eid2207.160326

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Funding

  1. National Institute of Allergy and Infectious Diseases of the National Institutes of Health (NIH) [K08AI110528]
  2. American Society of Tropical Medicine and Hygiene
  3. NIH [R01AI099631, U54AI65359, HHSN2722001000026C]
  4. Pediatric Dengue Vaccine Initiative [VE-1]

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Clinical manifestations of Zika virus, chikungunya virus, and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for these viruses.

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