Journal
JOURNAL OF PHARMACEUTICAL ANALYSIS
Volume 6, Issue 3, Pages 157-164Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpha.2016.01.004
Keywords
Ampicillin sodium; Surface molecularly imprinted polymers; Solid phase extraction; High performance liquid chromatography
Categories
Funding
- National Natural Science Foundation of China [81573391, 81173024]
- National Key Projects of China [812277802]
Ask authors/readers for more resources
Surface molecularly imprinted polymers (SMIPs) for selective adsorption of ampicillin sodium were synthesized using surface molecular imprinting technique with silica gel as a support. The physical and morphological characteristics of the polymers were investigated by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), elemental analysis and nitrogen adsorption-desorption test. The obtained results showed that the SMIPs displayed great adsorption capacity (13.5 mu g/mg), high recognition ability (the imprinted factor is 3.2) and good binding kinetics for ampicillin sodium. Finally, as solid phase extraction adsorbents, the SMIPs coupled with HPLC method were validated and applied for the enrichment, purification and determination of ampicillin sodium in real milk and blood samples. The averages of spiked accuracy ranged from 92.1% to 107.6%. The relative standard deviations of intra- and inter-day precisions were less than 4.6%. This study provides a new and promising method for enriching, extracting and determining ampicillin sodium in complex biological samples. (C) 2016 Xi'an Jiaotong University. Production and hosting by Elsevier B.V.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available