Journal
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
Volume 314, Issue 4, Pages H724-H732Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.00512.2017
Keywords
antibody; immunoblot analysis; immunoblotting; immunohistochemistry; rigor and reproducibility
Funding
- National Institutes of Health [HL-131834, HL-075360, HL-129823, HL-051971, GM-104357, GM-115428, GM-114833]
- Biomedical Laboratory Research and Development Service of the Veterans Affairs Office of Research and Development [5I01BX000505]
- Sarver Heart Center, University of Arizona
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL075360, P01HL051971, R01HL129823, R01HL131834] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [P30ES006694] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [U54GM114833, U54GM115428, P20GM104357] Funding Source: NIH RePORTER
- Veterans Affairs [I01BX000505] Funding Source: NIH RePORTER
Ask authors/readers for more resources
Antibody use is a critical component of cardiovascular physiology research, and antibodies are used to monitor protein abundance (immunoblot analysis) and protein expression and localization (in tissue by immunohistochemistry and in cells by immunocytochemistry). With ongoing discussions on how to improve reproducibility and rigor, the goal of this review is to provide best practice guidelines regarding how to optimize antibody use for increased rigor and reproducibility in both immunoblot analysis and immunohistochemistry approaches. Listen to this article's corresponding podcast at http://ajpheart.podbean.com/e/guidelines-on-antibody-use-in-physiology-studies/.
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