Journal
AMERICAN JOURNAL OF PATHOLOGY
Volume 188, Issue 10, Pages 2164-2176Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ajpath.2018.06.016
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Funding
- Wellbeing of Women Research training fellowship [RT414]
- Priory Foundation
- UK Medical Research Council [G1000709, MR/N026101/1, MR/R015325/1, MR/P026494/1, MR/N019075/1]
- Sport Aiding Medical Research for Kids (SPARKS) [17UCL01]
- Research Training Fellowship from Action Medical Research [GN2137]
- Action Medical Research grant [GN2075]
- [SPARKS 17UCL01]
- [MR/K02342X/1]
- [GOSHCCV1284]
- [Rosetrees M576]
- MRC [MR/P026494/1, MR/K02342X/1, G1000709, MR/R015325/1] Funding Source: UKRI
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Preterm birth is a serious global health problem and the Leading cause of infant death before 5 years of age. At least 40% of cases are associated with infection. The most common way for pathogens to access the uterine cavity is by ascending from the vagina. Bioluminescent pathogens have revolutionized the understanding of infectious diseases. We hypothesized that bioluminescent Escherichia coli can be used to track and monitor ascending vaginal infections. Two bioluminescent strains were studied: E. coli K12 MG1655-lux, a nonpathogenic laboratory strain, and E. coli K1 A192PP-lux2, a pathogenic strain capable of causing neonatal meningitis and sepsis in neonatal rats. On embryonic day 16, mice received intravaginal E. coli K12, E. coli K1, or phosphate-buffered saline followed by whole-body bioluminescent imaging. In both cases, intravaginal delivery of E. coli K12 or E. coli K1 led to bacterial ascension into the uterine cavity, but only E. coli K1 induced preterm parturition. Intravaginal administration of E. coli K1 significantly reduced the proportion of pups born alive compared with E. coli K12 and phosphate-buffered saline controls. However, in both groups of viable pups born after bacterial inoculation, there was evidence of comparable brain inflammation by postnatal day 6. This study ascribes specific mechanisms by which exposure to intrauterine bacteria leads to premature delivery and neurologic inflammation in neonates.
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